This study examined the influence of education, age, gender, and marital status on work ethic among Ghanaian professionals. Using a quantitative cross-sectional survey design, data were collected from 370 professionals across various sectors, including education, healthcare, finance, public administration, and engineering. Structured questionnaires measured respondents’ demographic characteristics and dimensions of work ethic, including diligence, discipline, and motivation. Data were analyzed using descriptive statistics, independent samples t-tests, and multiple regression analysis. The findings revealed that age, education, and marital status significantly predict variations in work ethic, with older, highly educated, and married professionals exhibiting higher levels of conscientiousness, discipline, and commitment. Gender, however, did not significantly influence overall work ethic, suggesting that male and female professionals demonstrate comparable professional behavior in Ghana. The study underscores the importance of considering demographic factors beyond gender when promoting ethical work practices and offers insights for designing policies that enhance professional productivity, fairness, and employee engagement in organizational settings.