This study investigated the effect of Zingiber officinale ethanol extract on serum electrolyte concentrations in male Wistar albino rats exposed to inflammation. Rats were randomly divided into five groups: Group A (blank control), Group B (negative control with induced inflammation and no treatment), Group C (standard control treated with a conventional anti-inflammatory drug), Group D (low-dose Zingiber officinale extract), and Group E (highdose Zingiber officinale extract). Serum levels of sodium (Na⁺), potassium (K⁺), chloride (Cl⁻), and calcium (Ca²⁺) were analyzed to assess the impact of treatments. The results revealed that inflammation significantly reduced serum electrolyte levels in the negative control group (B), with marked low sodium ion concentration (87 ± 0.008 mmol/L), low potassium ion concentration (2.2 ± 0.001 mmol/L), depleted level of chloride ion (57.40 ± 0.000 mmol/L), and low calcium ion concentration (2.2 ± 0.000 mg/dL) compared to the blank control group (A), all at p < 0.05. Administration of low-dose Zingiber officinale extract (Group D) showed no significant improvement in these electrolyte levels when compared to the negative control (p > 0.05). However, treatment with high-dose Zingiber officinale extract (Group E) significantly improved serum concentrations of Na⁺ (117 ± 0.003 mmol/L), K⁺ (5.6 ± 0.001 mmol/L), Cl⁻ (98.0 ± 0.004 mmol/L), and Ca²⁺ (4.4 ± 0.003 mg/dL), indicating a dose-dependent restorative effect. These findings inferred that high-dose Zingiber officinale ethanol extract may have restored electrolyte balance in inflammatory conditions, potentially serving as a natural therapeutic agent.